PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. \u003ci\u003e\u003cstrong\u003ePCR: Methods Express\u003c/strong\u003e\u003c/i\u003e describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including:\u003cbr\u003e\n\u003cbr\u003e\n*real time qRT-PCR\u003cbr\u003e\n*rapid generation of gene targeting constructs\u003cbr\u003e\n*PCR multiplexes\u003cbr\u003e\n*PCR-based mutagenesis\u003cbr\u003e\n*identification of microdeletions and microduplications\u003cbr\u003e\n\u003cbr\u003e\n*DNA methylation analysis\u003cbr\u003e\n*forensic genetic DNA typing\u003cbr\u003e\n*genotyping\u003cbr\u003e\n*identification of mutations in single cells\u003cbr\u003e\n*whole genome amplification\u003cbr\u003e\n*diagnosis of infectious diseases\u003cbr\u003e\n*inverse PCR-based RFLP\u003cbr\u003e\n\u003cbr\u003e\nThis book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. \u003ci\u003e\u003cstrong\u003ePCR: Methods Express\u003c/strong\u003e \u003c/i\u003eis an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator. \u003cbr\u003e
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