This brief introduces the classification and mechanism of density gradient ultracentrifugation (DGUC) method with rich examples showing the versatility of such an efficient separation technique. It also gives a strict mathematical description and a computational optimization model to predict the best separation parameters for a given colloidal system. The concept of âLab in a tubeâ is proposed in the last chapter, which allows the size-property relationship investigation, synthetic optimization and reaction/assembly mechanism exploration etc.
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